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Islets were then washed with the Cholesterol Detection wash buffer, incubated for a minimum of 1 hour in Filipin III diluted in the Cholesterol Detection Assay buffer, and washed again in wash buffer before immediate imaging on a Zeiss LSM-780 inverted confocal laser-scanning microscope from the Facility for Imaging by Light Microscopy (FILM) at Imperial College London, with a 63X objective and a laser excitation wavelength of 340-380 nm and emission of 385-470 nm

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Recently, inactivation of the polycomb repressive complex 2 (PRC2), induced by inactivating mutations in two of its critical constituents SUZ12 and EED , was reported in a large subset of malignant peripheral nerve sheath tumors

Glutathione is a primary detox antioxidant that enhances liver enzyme function and supports toxin removal pathways